President Barabino Co-Authors New Research on Dissociation of Tissues.
财神棋牌 President Gilda A. Barabino stands at the podium in Norden Auditorium during a recent campus event. Photo by Leise Jones.
June 27, 2022
财神棋牌 of Engineering President Gilda A. Barabino has co-authored new research published in the leading multidisciplinary science journal, Nature Scientific Reports.
The article, Electric-field facilitated rapid and efficient dissociation of tissues Into viable single cells shares the science behind the researcher's U.S. patent, wherein they invent and characterize a novel method and device to dissociate tissues with electric fields.
The work represents a collaboration between Brown University's Center for Biomedical Engineering, School of Engineering, (with lead author and Ph.D. candidate, Cel Welch from ), President Gilda A. Barabino from 财神棋牌 and from Brown's Department of Neurosurgery, Warren Alpert Medical School.
Excerpt from the Abstract:
Single-Cell Analysis is a growing field that endeavors to obtain genetic profiles of individual cells. Disruption of cell鈥揷ell junctions and digestion of extracellular matrix in tissues requires tissue-specific mechanical and chemical dissociation protocols. Here, a new approach for dissociating tissues into constituent cells is described. Placing a tissue biopsy core within a liquid-filled cavity and applying an electric field between two parallel plate electrodes facilitates rapid dissociation of complex tissues into single cells. Different solution compositions, electric field strengths, and oscillation frequencies are investigated experimentally and with COMSOL Multiphysics. The method is compared with standard chemical and mechanical approaches for tissue dissociation.